Kingfisher duo prime bdz) files can be found under the Documents>Product literature section. Genomic DNA was extracted from 5 x 105 HeLa-S3 cells on the KingFisher Duo Prime (#5400110) with the KingFisher Cell and Tissue DNA Kit (#97030196). KingFisher Duo Prime works in standard 24- and 96-plate formats and typically there is a need to use several pipettes of different volume settings to fill the reagents on the plate and the elution strip. Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher. . It provides excellent reproducibility with minimal hands-on time, and has a small Do not eat, drink, smoke, 1. Thermo Scientific BindIt software is included with KingFisher Duo Prime and Flex instruments. The main differences are throughput, the number of samples per run, and the way the models handle the plates and process. Package includes the KingFisher Duo Prime Purification System and SmartSmart Orientation. com. Typically any warranty, service contract or license for the equipment is not transferable but may be under certain circumstances and will be Aug 23, 2024 · Used THERMO FISHER KingFisher Duo Prime DNA Extractor For Sale - DOTmed Listing #4377106: Description The Thermo KingFisher Duo Prime was manufactured 11/21/2019. The KingFisher Duo Prime instrument contains a mercury lamp with peak emission around 255 nm. Ideal for laboratories requiring nucleic acid purification and is easily modified for use in proteomics and cell isolation. Not for use in diagnostic procedures. KingFisher Duo Prime is an affordable choice for academic or small labs. Kits are available to recover total RNA, microRNA (miRNA), mRNA, genomic DNA, cell-free The KingFisher Duo Prime system supports a variety of analytes and sample types for a wide range of protocols from trusted reagents. During the individual steps, the plates are kept stationary, and the only moving assembly is the processing head with tip comb and magnetic rods. Optimize your performance using Thermo Scientific™ KingFisher Plastics designed specifically for use with the KingFisher Flex, Duo Prime, Presto and Apex instruments. The standard Cell and Tissue protocol was customized in three ways: Walk-Away, Dispense-1 and Dispense-2 (Figure 1). Unscrew and 4. 2 ml. KingFisher™ Plastics for 96 deep-well format. KingFisher instruments utilize disposable plastic plates and tip combs made of polypropylene, which are ideal for magnetic particle processing due to their low binding affinity for biomolecules. It offers consistent extraction and purification of DNA, RNA, proteins, and cells, and includes an ultraviolet lamp for decontamination. View online or download Thermo scientific KingFisher Duo User Manual KingFisher Duo/Duo Prime: The KingFisher Duo and Duo Prime instruments accept all characters in the filename. Remove and save 2. collection tube . Available in Elution Strip Nov 2, 2020 · Subscribed 12 5. Available in The Thermo Scientific Kingfisher Duo Prime offers efficient and reliable nucleic acid purification, making it an ideal choice for labs needing high-throughput processing capabilities. Kingfisher Duo Prime | Government Scientific SourceSpecifications Inventory Number: FSC14387700EA Part Number: 14387700 Supplier: Fisher Scientific Supplier's Lead Time: 2 to 3 Weeks Quantity: 1 per EA Contract Availability: Country of Origin: Finland Collection EfficiencyofParticles: >=95% Computer Interface: USB Cooling Temperature: 4 to 75°C in RT (elution strip) Depth English : 18. What is the difference between the KingFisher Duo and the KingFisher Duo Prime Purification Systems? The KingFisher Duo Prime Purification System has the following additional features compared to the KingFisher Duo Purification System: • Improved safety shield design • UV lamp • Handheld barcode reader option • Additional language options • Optimized elution strip block for better KingFisher Duo Prime 30 - 5000 μL まで6 または12 サンプル同時精製(連続して最高24サンプル) 専用の24または96ディープウェルプレートを使用 冷却機能付きでゲノムDNAやタンパク質など多彩なアプリケーションに対応 殺菌用UVランプ搭載 The Thermo Scientific KingFisher Duo Prime is the most versatile compact benchtop sample preparation instrument in the lab for 6 or 12 samples per run. Fill saliva to 3. What is the difference between the KingFisher Duo and the KingFisher Duo Prime Purification Systems? The KingFisher Duo Prime Purification System has the following additional features compared to the KingFisher Duo Purification System: • Improved safety shield design • UV lamp • Handheld barcode reader option • Additional language options • Optimized elution strip block for better The KingFisher Duo Prime system is a flexible system for sample processing that offers highly versatile, automated magnetic-particle processing for DNA/RNA, protein, and cell purification from a wide range of sources. A processing volume of 30–5,000 μl with the following formats: 96 deep well Plate 24 deep well Plate KingFisher™ Plastics for 96 deep-well format. Dispose of all microplates, strips and tip combs as biohazardous waste. Available in Tip Comb Thermo Scientific KingFisher Duo Prime Purification System from Thermo Scientific™. Each sample type and application require different chemistries to effectively extract the target KingFisher Duo Prime and KingFisher Duo Purification of 6 or 12 samples in one run. Nov 21, 2019 · Description Thermo KingFisher Duo Prime The Thermo KingFisher Duo Prime was manufactured 11/21/2019. Available in The KingFisher Duo Prime system is a flexible system for sample processing that offers highly versatile, automated magnetic-particle processing for DNA/RNA, protein, and cell purification from a wide range of sources. Thermo Scientific KingFisher systems are designed to isolate DNA, RNA, and proteins from a variety of starting materials, including cell-free body fluids, blood, bacteria, cell cultures, tissue, and plant samples. 0. com The Thermo Scientific KingFisher Duo Prime is the most versatile compact benchtop sample preparation instrument in the lab for 6 or 12 samples per run. The step is carried out with a dedicated post-PCR KingFisher Duo Prime to guarantee tha the samples are pooled and sequenced. DNA quality control of fragment size, purity and concentration is performed severa Jun 6, 2023 · Introducing an automated nucleic acid purification platform in the Genomics Core - KingFisher Duo Prime System. KingFisher Duo Prime 核酸・タンパク質・細胞自動抽出・精製装置 Thermo ScientificTM KingFisherTM Duo Prime核酸・タンパク質・細胞自動抽出・精製装置は、磁気ビーズを使用して、一度に最大12 サンプルおよび6サンプルの自動核酸抽出およびタンパク質精製が可能です。 KingFisher™ Duo Prime Purification System with SmartStart. It is encouraged that you inspect this equipment if possible. This state-of-the-art technology enables efficient extraction of DNA and RNA from various sample types with high purity and yield, streamlining workflows and reducing contamination risks. It provides consistent extraction and purification of DNA, RNA, proteins and cells and includes an ultraviolet lamp for decontamination. KingFisher is a flexible system for different types of sample processing needs which offers highly versatile, automated magnetic-processing for DNA/RNA, protein, and cell purification from virtually any source. Available in Tip Comb The KingFisher Duo Prime instrument works with our optimized KingFisher Pure DNA/RNA purification kits, plastic consumables and Thermo ScientificTM BindItTM software to provide a complete, highly sensitive automated system for your purification workflow. The KingFisher Duo Prime system is a flexible system for sample processing that offers highly versatile, automated magnetic-particle processing for DNA/RNA, protein, and cell purification from a wide range of sources. With its dual-head design, it can process up to 96 samples simultaneously, making it suitable for various applications in KingFisher instrument–ready reagents Reduce your pipetting steps with prefilled reagent plates for Thermo ScientificTM KingFisherTM Duo Prime and Thermo ScientificTM KingFisherTM Flex instruments. The Thermo Scientific KingFisher Duo Prime is the most versatile compact benchtop sample preparation instrument in the lab for 6 or 12 samples per run. This Thermo KingFisher Duo Prime KingFisher Duo Prime The Thermo Scientific KingFisher Duo Prime is the most versatile compact benchtop sample preparation instrument in the lab for 6 or 12 samples per run. Available in Tip Comb Optimize your performance using Thermo Scientific™ KingFisher Plastics designed specifically for use with the KingFisher Flex, Duo Prime, Presto and Apex instruments. com or another major laboratory supplier. 0 Kit reagents are available as automation-friendly stand-alone reagents. 33MB) Choose a promotion: KingFisher Duo Prime system The Thermo ScientificTM KingFisherTM Duo Prime Purification System offers an economical option for automated nucleic acid extraction and protein purification from up to 12 samples at a time and 24 samples per load using magnetic beads. Both the yield and quality of Thermo scientific KingFisher Duo Pdf User Manuals. Optimize your performance using Thermo Scientific™ KingFisher consumables designed specifically for use with the KingFisher Flex, Duo Prime, and Presto instruments. The Thermo Scientific KingFisher Duo Prime is the most versatile compact benchtop sample preparation instrument in the lab for 6 or 12 samples per run. 0 isolation reagents come prefilled into the plates that go in the KingFisher instrument. Search for used Kingfisher. The instrument is intended for automated transfer and processing of magnetic particles in a microplate format. Thermo Scientific KingFisher Duo Prime purification system provides convenient and reproducible DNA/RNA extraction and protein isolation. 0-alpha-20201231-10-g1236 Ocr_autonomous true Ocr_detected_lang en Ocr_detected_lang_conf 1. Find additional tips, troubleshooting help, and The complete user manual for the KingFisher Duo Prime instrument is available on the KingFisher User Manuals CD. 0 Prefilled Plates are developed for scalable, rapid purification of high-quality DNA from a variety of sample matrices. Thermo Scientific KingFisher Duo Prime Purification System from Thermo Scientific™. The best place to download KingFisher Flex and KingFisher Duo Prime protocols for a particular MagMAX kit is the relevant MagMAX kit product page. This Thermo KingFisher Duo Prime is being sold “as is, where is” unless otherwise noted. Available in Tip Comb The Thermo Scientific KingFisher Duo Prime is the most versatile compact benchtop sample preparation instrument in the lab for 6 or 12 samples per run. The adjustable tip spacing KingFisher Duo Prime The KingFisher Duo Prime system automa-tes purification processes for research and routine laboratories working with a smaller number of samples. KingFisher™ instruments reduce your hands-on time while maintaining high yields KingFisher Duo Prime The Thermo Scientific KingFisher Duo Prime is the most versatile compact benchtop sample preparation instrument in the lab for 6 or 12 samples per run. 0 line (not discard the funnel the tube, then mix or chew gum for at least attach the funnel including bubbles). Maximum capacity hundreds of samples per day. Available in Combi Pack This user manual is written for the actual end user, for example, research scientist or laboratory technician. The barcode sequences allow for mul iple sample analyses on a single run. s additional PCR reaction components. The Bindlt script protocol (. Thermo Scientific KingFisher Duo Prime Brief User Manual 17 3 Operation Starting KingFisher Duo Prime 7. # 製品の概要 コンパクトな Thermo Scientific KingFisher Duo Prime は、小規模から中規模のスループットのラボで、核酸やタンパク質の精製を自動化するための柔軟で使いやすいシステムです。 KingFisher Duo Prime と MagMAX 核酸抽出・精製キットと組み合わせることで、さまざまなサンプル量やサンプル Thermo Scientific KingFisher Plastics for 96 deep-well format from Thermo Scientific™. The KingFisher Duo Prime (low/medium throughput), meant for small labs and individual researchers, processes up to 12 samples per run The KingFisher Flex (medium/high throughput), the standalone laboratory workhorse, processes up to 96 samples per run and is ideal for high-throughput SARS-CoV-2 testing Do you have data that shows how the UV lamp of the KingFisher Duo Prime instrument gets rid of bacterial contamination? Yes we have an application note that demonstrates the lethal effect of UVC irradiation on bacterial growth and deactivation of human gDNA, "Decontamination of the KingFisher Duo Prime processing chamber using UV light". KingFisher Duo Prime is the most versatile compact benchtop sample preparation instrument and provides a flexible and convenient solution for automating the purification of nucleic acids and proteins. DNA purified with this kit can be used in a broad range of molecular biology downstream applications, such as sequencing, genotyping, and qPCR. For Research Use Only. This Thermo KingFisher Duo Prime # 製品の概要 コンパクトな Thermo Scientific KingFisher Duo Prime は、小規模から中規模のスループットのラボで、核酸やタンパク質の精製を自動化するための柔軟で使いやすいシステムです。 KingFisher Duo Prime と MagMAX 核酸抽出・精製キットと組み合わせることで、さまざまなサンプル量やサンプル Thermo Scientific KingFisher Plastics for 96 deep-well format from Thermo Scientific™. 0), using the time-course protocol outlined in Table 3, which is available for download via the Thermo ScientificTM AppsLab Library of Analytical Applications. The dimensions of the plate conform to SBS standards, and the unique V-bottom shape of the wells make it compatible with popular bead moving instruments such as the KingFisher Flex, KingFisher Apex, KingFisher Duo Prime, and KingFisher Presto. This compact prime purification system has excellent reproducibility and minimal hands-on time, freeing you up for other tasks. This manual provides information on the Thermo Scientific™ KingFisher™ Duo Prime magnetic particle processor, including the installation and operating instructions. Purification with the KingFisherTM Duo Prime or KingFisherTM mL instrument Required materials not supplied Unless otherwise indicated, all materials are available through thermofisher. your saliva sample. (2:49) ミドルスループットに対応するシステム KingFisher Duo Prime 30 - 5,000 μLまで6または12 サンプル同時精製(連続して最高24サンプル) 専用の24 または96ディープウェルプレートを使用 抽出したサンプルの一時的な冷却保管が可能 殺菌用UVランプ搭載 KingFisher Duo Prime系统 Thermo Scientific KingFisher Duo Prime纯化系统提供了经济实惠的自动化核酸提取和蛋白质纯化方案,一次可运行至多12 个样本, 使用磁珠可以一次加载24 个样本。 The KingFisher Duo Prime instrument works with our optimized KingFisher Pure DNA/RNA purification kits, plastic consumables and Thermo ScientificTM BindItTM software to provide a complete, highly sensitive automated system for your purification workflow. The 96 Deep Well Plate features durable, clear polypropylene construction and a capacity of 2. cfDNA MAGNETIC BEAD PURIFICATION ON THE KINGFISHER This protocol starts with AFA-conditioned plasma that was obtained after Step E in Section-4 in the truXTRAC® cfDNA Kit – Magnetic Bead Purification Manual. To help save time and increase reproducibility, combine with KingFisher instruments for automated purification. Secure the cap on use tobacco products, the cap , then the 2. Consistent extraction and purification of DNA, RNA, protein, and cells. It provides excellent reproducibility with minimal hands-on time, and has a small Thermo Scientific™ KingFisher™ 12-Tip Comb For use with KingFisher Duo Prime Only COMB, 12-TIP KING FISHER F/96 DEEP-WELL PLATE (50/CS) Thermo Scientific KingFisher Duo Primeは、1回のランで6または12サンプルを処理する、ラボでもっとも汎用性の高いコンパクトなベンチトップ型サンプル調製機器です。DNA、RNA、タンパク質、および細胞の一貫した抽出と精製を実現し、汚染除去用のUVランプを備えています。KingFisher Duo Primeは、学術機関 Features of the KingFisher Duo Prime system Isolates DNA, RNA and proteins from a variety of starting materials including cell-free body fluids, blood, bacteria, cell cultures, tissue and plant samples; with volumes ranging up to 5 mL Thermo Scientific™ KingFisher™ Duo Prime Purification System Purchase one KingFisher Duo Prime and get 12 plastic consumable packs at no additional cost! Limit 5 offers per lab. Available in Combi Pack Kingfisher Duo Prime is the most versatile compact benchtop sample prep instrument in the lab for 6 or 12 samples per run. The KingFisher Duo Prime is an affordable choice for academic or small labs. com for assistance. CAUTION Remove any plates, strips or tip combs still in the instrument. • Click the "Category" pulldown to select the instrument folder you want to load the script to. The software makes it easy to create, download, run, modify, and store protocols for your KingFisher applications. Features of the KingFisher Duo Prime system Isolates DNA, RNA and proteins from a variety of starting materials including cell-free body fluids, blood, bacteria, cell cultures, tissue and plant samples; with volumes ranging up to 5 mL Reap high yields of quality nucleic acids from a variety of starting materials with the Thermo Scientific™ KingFisher™ Duo Prime purification system. Thermo Scientific KingFisher Duo is a low- to medium-throughput purification system consisting of the KingFisher® Duo magnetic particle processor instrument, optimized DNA/RNA purification kits, plastics consumables and the BindIt software, providing a highly sensitive automated workflow. KingFisher instrument BindIt software scripts for all sample types and both Flex and Duo Prime systems can be downloaded below and installed onto the KingFisher instruments. Possibility to run two purification methods sequentially without interruption, raising the throughput to 24 samples. The manual provides detailed instructions on using the instrument for automated magnetic particle processing, including safety symbols, maintenance procedures, and troubleshooting. For instructions on the BindIt Software, see the BindIt Software for KingFisher Instruments CD. Both the yield and quality of Nov 21, 2019 · Description Thermo KingFisher Duo Prime The Thermo KingFisher Duo Prime was manufactured 11/21/2019. Reap high yields of quality nucleic acids from a variety of starting materials with the Thermo Scientific™ KingFisher™ Duo Prime purification system. KingFisher Duo Prime demo video This short video demonstrates how the KingFisher Duo Prime can help you achieve high-quality, high-yield sample preparation with fewer manual steps. Read the user manuals in their entirety before operating the instrument. Working with higher throughput sample numbers? The same MagMAX DNA Multi-Sample Ultra 2. Oct 19, 2023 · Thermo Scientific™ KingFisher Duo Prime Brief User Manual Update: 19 October, 2023 Thermo Scientific™ KingFisher Duo Prime Brief User Manual. Available in 96 Deep Well plate, v-bottom Thermo Scientific KingFisher Duo Prime Purification System from Thermo Scientific™. This compact instrument is ideal for laboratories requiring nucleic acid purification, and is easily modified for use in proteomics and cell isolation. The head The KingFisher Duo Prime instrument works with our optimized KingFisher Pure DNA/RNA purification kits, plastic consumables and Thermo ScientificTM BindItTM software to provide a complete, highly sensitive automated system for your purification workflow. 1 in Automated extraction of nucleic acids, cells, exosomes or proteins is made possible by combining the right magnetic bead-based kit with a KingFisher sample purification system. Both the yield and quality of The Thermo Scientific KingFisher Duo Prime is the most versatile compact desktop sample preparation in the lab for 6 or 12 samples per run. This compact instrument is ideal for laboratories requiring nucleic acid Digestion was performed using the Kingfisher Duo Prime purification system with BindIt software (version 4. Our kits are recommended for viral nucleic acid isolation for SARS-CoV-2 and other other Thermo Scientific KingFisher Duo Prime Purification System from Thermo Scientific™. 6K views 4 years ago KingFisher Duo Prime: How to run a demo protocolmore The Thermo Scientific KingFisher Duo Prime is the most versatile compact benchtop sample preparation instrument in the lab for 6 or 12 samples per run. Micro-organisms are destroyed due to the UVC radiation causing DNA lesions, which block transcription and replication with lethal consequences. by inverting the tube 30 minutes before giving securely to the five times. Product description The Thermo ScientificTM KingFisherTM Duo-Ready DNA Ultra 2. Reap high yields of quality DNA, RNA and proteins from a variety of starting materials with the KingFisher Duo Prime purification system. KingFisher Duo Prime Purification Systems: How to transfer files with USB Thermo Fisher Scientific 130K subscribers Subscribe The Thermo Scientific KingFisher Duo Prime is the most versatile compact benchtop sample preparation instrument in the lab for 6 or 12 samples per run. (Do not reuse). The KingFisher Duo Prime system supports a variety of analytes and sample types for a wide range of protocols from trusted reagents. Carry out magnetic particle processing with Kingfisher Duo and Kingfisher Duo Prime Consumables, specially designed combs and microplates optimized for performance. This document aims to give you the information you need for: • Reviewing safety precautions • Installing the 4 days ago · Quick step-by-step video showing the steps for using the KingFisher Duo Prime with the MagMAX Viral/Pathogen Nucleic Acid Isolation kit for SARS-COV-2 sample preparation. "MLS" indicates that the material is available from fisherscientific. Now, MagMAX DNA Multi-Sample Ultra 2. The tip combs which protect the magnets during processing are compatible with the plates. Additional magnet heads and heating blocks are offered here for separate purchase. Find Thermo Fisher Scientific and Kingfisher for sale on Machinio. The KingFisher Duo Prime magnetic particle processor (see Figure 1) is intended for professional research use by trained personnel. Using revolutionary magnetic particle separation technology, this system provides excellent reproducibility and quality. Available in Tip Comb Thermo Scientific KingFisher Plastics for 96 deep-well format from Thermo Scientific™. The Thermo Kingfisher Duo Prime Purification System provides your lab with quality nucleic acids of high yields. Can I scale up the MagMAX kit reaction volumes? If so, how should I do this? Subscribed 1 698 views 4 years ago KingFisher Duo Prime How to do a tip comb checkmore This compact purification instrument provides high quality yields of nucleic acids (DNA/RNA) and proteins with excellent reproducibility and minimal hands-on time. See all of our promotional offers here (PDF, 5. The KingFisher Duo Prime instrument works with our optimized KingFisher Pure DNA/RNA purification kits, plastic consumables and Thermo ScientificTM BindItTM software to provide a complete, highly sensitive automated system for your purification workflow. This protocol guides users through automated MagMAX Viral/Pathogen kits and reagents use magnetic bead-based technology to purify high-quality nucleic acid from a range of research sample types both manually and through automation. Available kits, individual reagents, and downloadable protocols enable sample preparation that is easy and efficient. Available in Each Thermo Fisher KingFisher Duo Prime system: How to run a demo protocol Thermo Fisher Scientific 125K subscribers 14 Use our selection tool to find the right magnetic bead-based kit for your automated sample preparation. Download the KingFisher Duo The Thermo Scientific KingFisher Duo Prime is the most versatile compact benchtop sample preparation instrument in the lab for 6 or 12 samples per run. When the run is finished, remove the plate(s) and strip(s) according to the protocol request. KingFisher Duo Prime是功能全面的台式核酸蛋白纯化仪，每次运行可处理6或12个样品，可用于磁珠操作、处理、分离，RNA 或 DNA 自动提取、分离和纯化工作流程。并提供用于净化的紫外灯。 Additional accessories are available for the KingFisher system. Shop Thermo. King Fisher Duo Prime Magnetic Particle Processor The KingFisher Duo magnetic particle processor has room for two plates and two elution strips. Both the yield and quality of Oct 13, 2017 · Optimize and automate your nucleic acid and protein purification with Thermo Scientific™ KingFisher™ systems. The complete user manual for the KingFisher Duo Prime instrument is available on the KingFisher User Manuals CD. The KingFisher Duo Prime (low/medium throughput), meant for small labs and individual researchers, processes up to 12 samples per run The KingFisher Flex (medium/high throughput), the standalone laboratory workhorse, processes up to 96 samples per run and is ideal for high Mar 1, 2021 · KingFisher Duo Prime Brief User Manual Topics manualzilla, manuals, Collection manuals_contributions; manuals; additional_collections Addeddate 2021-03-01 16:28:18 Identifier manualzilla-id-5674307 Identifier-ark ark:/13960/t1fk35240 Ocr tesseract 5. Reap high yields of quality nucleic acids from a variety of starting materials with the Thermo Scientific KingFisher Duo Prime purification system The Thermo Scientific KingFisher Duo Prime is a versatile, automated nucleic acid purification system designed for high throughput, size-exclusion-based, magnetic bead purification of nucleic acids from various sources. MagMAX, Dynabeads, and SMART Digest kits can purify nucleic acids, proteins, cells, exosomes, and virus from a wide range of sample types and are optimized for automated Kingfisher purification systems. Not anymore! Thermo Scientific KingFisher deep-well 96-plate and KingFisher Duo Prime elution strips can easily be filled using the E1-ClipTip Equalizer pipette. It provides excellent reproducibility with minimal hands-on time, and has a small View and Download Thermo Scientific KingFisher Duo user manual online. The system delivers high quality yields with excellent reproduci-bility and reliability. KingFisher™ Duo Prime精製システム. Compare KingFisher elution strip for 12 pin magnet (for Duo Prime only) from Thermo Fisher Scientific on Biocompare. KingFisher Duo laboratory equipment pdf manual download. Combining the KingFisher Duo Prime system with Dynabeads or MagMAX nucleic acid extraction kits allows a diverse range of sample KingFisher™ Plastics for 96 deep-well format. Available in Tip Comb KingFisher Duo/Duo Prime: The KingFisher Duo and Duo Prime instruments accept all characters in the filename. Aug 12, 2025 · Ensure the KingFisher Duo is installed according to the user manual, which includes safety precautions, installation steps, and operational guidelines. This automated system streamlines workflows, saving time and increasing productivity. The KingFisher Duo Prime instrument comes with a 12-pin magnet head for 96 deep well applications, a 6-pin magnet head for 24 deep well applications, and heating blocks. qbf ngbf qlvyh hmexyi yipikuvz tawff ofjfc wixmt fpjy qxm mcrnb wuf xct igujn yguuinu